Article
Identification of the activating region of catabolite gene activator protein (CAP): isolation and characterization of mutants of CAP specifically defective in transcription activation.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1993
Zhou Y, Zhang X, Ebright R H
Abstract excerpt
We have isolated 21 mutants of catabolite gene activator protein (CAP) defective in transcription activation at the lac promoter but not defective in DNA binding. The amino acid substitutions in the mutants map to a single region of CAP: amino acids 156-162. As assessed in vitro, the substituted...
Topics
- Base Sequence
- Cyclic AMP Receptor Protein
- DNA
- DNA-Directed RNA Polymerases
- Lac Operon
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Peptide Fragments
- Promoter Regions, Genetic
- Structure-Activity Relationship
- Transcriptional Activation