Article
Catabolite gene activator protein (CAP) is not an "acidic activating region" transcription activator protein. Negatively charged amino acids of CAP that are solvent-accessible in the CAP-DNA complex play no role in transcription activation at the lac promoter.
The Journal of biological chemistry - 25 Apr 1992
Zhang X, Zhou Y, Ebright Y W, Ebright R H
Abstract excerpt
It has been suggested that the catabolite gene activator protein (CAP) uses an "acidic activating region" transcription activation mechanism and that Glu171 of CAP is the critical amino acid of the "acidic activating region" of CAP (Irwin, N., and Ptashne, M. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 8315-8319). In this paper, we show, contrary to the previously published report, that substitution of Glu171 of CAP...
Topics
- Amino Acids
- Cyclic AMP Receptor Protein
- Escherichia coli
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Solvents
- Transcription, Genetic