Article
Creeping yeast: a simple, cheap and robust protocol for the identification of mating type in Saccharomyces cerevisiae.
FEMS yeast research - 26 Apr 2022
Arras Samantha D M, Hibbard Taylor R, Mitsugi-McHattie Lucy, Woods Matthew A, Johnson Charlotte E, Munkacsi Andrew, Hermann-Le Denmat Sylvie, Ganley Austen R D
Abstract excerpt
Saccharomyces cerevisiae is an exceptional genetic system, with genetic crosses facilitated by its ability to be maintained in haploid and diploid forms. Such crosses are straightforward if the mating type/ploidy of the strains is known. Several techniques can determine mating type (or ploidy), but all have limitations. Here, we validate a simple, cheap and robust method to identify S. cerevisiae mating types....
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