Article
Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci.
Biochemistry - 1 Nov 1994
Schlake T, Bode J
Abstract excerpt
Using the FLP/FRT system for site-specific recombination and the wild-type recognition site (FRT) in conjunction with certain mutant FRT sites, it becomes possible to provoke, with high yield, a double-reciprocal crossover event in cultured mammalian cells. It is demonstrated that this technology...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Line
- Cricetinae
- DNA
- DNA Nucleotidyltransferases
- Kidney
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Polymerase Chain Reaction
- Recombination, Genetic