Article
Expression of Flp Protein in a Baculovirus/Insect Cell System for Biotechnological Applications.
The protein journal - 1 Aug 2017
Jensen Ida S, Inui Ken, Drakulic Srdja, Jayaprakash Sakthidasan, Sander Bjoern, Golas Monika M
Abstract excerpt
The Saccharomyces cerevisiae Flp protein is a site-specific recombinase that recognizes and binds to the Flp recognition target (FRT) site, a specific sequence comprised of at least two inverted repeats separated by a spacer. Binding of four monomers of Flp is required to mediate recombination between two FRT sites. Because of its site-specific cleavage characteristics, Flp has been established as a genome...
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