Article
A simple analysis system for the estimation of recombination efficiency using fluorescence-activated cell sorting.
Journal of biotechnology - 10 Jan 2007
Kim Min Soo, Kim Won Hee, Lee Gyun Min
Abstract excerpt
A simple and accurate analysis system for the estimation of recombination efficiency in vivo using fluorescence-activated cell sorting (FACS) was designed and was subsequently used to compare the efficiency of recombination related to different spacer mutants. F(3) and F(5) mutant sequences were used for Flpe-mediated cassette exchange, and m2 and lox2272 mutant sequences were used for Cre-mediated cassette...
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