Article
Physical mapping of two temperature-sensitive adenovirus mutants affected in the DNA polymerase and DNA binding protein.
Virus genes - 1 Jun 1990
Roovers D J, Young C S, Vos H L, Sussenbach J S
Abstract excerpt
We have determined the exact nature of two thermosensitive (ts) adenovirus mutants, H5ts19 and H5ts149, which map to different genes in the E2 transcription unit. The H5ts19 mutation appears to stem from a single base-pair change of A-T to G-C at position 1840 (numbering as in ref. 1), corresponding to codon 154 of the gene coding for DBP. This results in a glutamine-to-arginine change in the amino-terminal...
Topics
- Adenoviridae
- Base Sequence
- DNA, Viral
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Genetic Markers
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Nucleotide Mapping