Article
Somatic cell genetic and biochemical characterization of cell lines resulting from human genomic DNA transfections of Chinese hamster ovary cell mutants defective in sterol-dependent activation of sterol synthesis and LDL receptor expression.
Somatic cell and molecular genetics - 1 May 1994
Hasan M T, Chang C C, Chang T Y
Abstract excerpt
We have isolated several non-leaky mutant Chinese hamster ovary (CHO) cell clones (M4, M19, and M21) requiring cholesterol and unsaturated fatty acid for growth. These mutants belong to the same complementation group as the mutant M1 cells previously reported from this laboratory. M19 cells rever...
Topics
- Amphotericin B
- Animals
- CHO Cells
- Cell Line
- Clone Cells
- Cricetinae
- Cricetulus
- Culture Media
- DNA
- Gamma Rays
- Gene Expression Regulation
- Genetic Complementation Test
- Genetic Linkage
- Humans
- Hybrid Cells
- Hydroxymethylglutaryl CoA Reductases
- Hydroxymethylglutaryl-CoA Synthase
- Mutagenesis