Isolation of cholesterol-requiring mutant Chinese hamster ovary cells with defects in cleavage of sterol regulatory element-binding proteins at site 1.

doi:10.1074/jbc.273.43.28261

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Article

Isolation of cholesterol-requiring mutant Chinese hamster ovary cells with defects in cleavage of sterol regulatory element-binding proteins at site 1.

The Journal of biological chemistry - 23 Oct 1998

Rawson R B, Cheng D, Brown M S, Goldstein J L

Abstract excerpt

The synthesis and uptake of cholesterol requires transcription factors designated sterol regulatory element-binding proteins (SREBPs). SREBPs are bound to membranes in a hairpin orientation with their transcriptionally active NH2-terminal segments facing the cytosol. The NH2-terminal segments are...

Topics

Acetates
Animals
Biological Transport
CCAAT-Enhancer-Binding Proteins
CHO Cells
Cholesterol
Cricetinae
DNA-Binding Proteins
Gene Dosage
Gene Expression Regulation
Genetic Complementation Test

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Lipoproteins, LDL

Mutation

Nuclear Proteins

Protein Processing, Post-Translational

Sterol Regulatory Element Binding Protein 1

Sterol Regulatory Element Binding Protein 2

Transcription Factors

Source

Open in PubMed

Identifiers

PMID: 9774448
DOI: 10.1074/jbc.273.43.28261

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9774448-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Isolation of cholesterol-requiring mutant Chinese hamster ovary cells with defects in cleavage of sterol regulatory element-binding proteins at site 1.PMID 9774448Read ↗

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Autocatalytic processing of site-1 protease removes propeptide and permits cleavage of sterol regulatory element-binding proteins.Semantic proximity Read ↗

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