Article
Direct evidence for ligand-induced internalization of the yeast alpha-factor pheromone receptor.
Molecular and cellular biology - 1 Nov 1994
Schandel K A, Jenness D D
Abstract excerpt
When Saccharomyces cerevisiae a cells bind alpha-factor pheromone, the ligand is internalized and its binding sites are lost from the cell surface in a time-, energy-, and temperature-dependent manner. This report presents direct evidence for alpha-factor-induced internalization of cell surface receptors. First, membrane fractionation on Renografin density gradients indicated that the alpha-factor receptors were...
Topics
- Biological Transport, Active
- Cell Membrane
- Endocytosis
- Endopeptidases
- Genes, Fungal
- Kinetics
- Mating Factor
- Mutation
- Peptides
- Receptors, Mating Factor
- Receptors, Peptide