Article
Analysis of diversity of T cell antigen receptor genes using polymerase chain reaction and sequencing gel electrophoresis.
Journal of immunological methods - 14 Oct 1994
Yurovsky V V, Schulze D H, White B
Abstract excerpt
A sensitive, highly resolvable, and quantitative method was designed to analyse the diversity of polymerase chain reaction (PCR)-amplified transcripts which possess length polymorphism. A reverse transcriptase-PCR technique was used to amplify rearranged T cell antigen receptor (TCR) transcripts isolated from human blood. Oligonucleotide primers specific for conserved TCR V and C region sequences were used in...
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