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Article

Analysis of diversity of T cell antigen receptor genes using polymerase chain reaction and sequencing gel electrophoresis.

Journal of immunological methods - 14 Oct 1994

Yurovsky V V, Schulze D H, White B

Abstract excerpt

A sensitive, highly resolvable, and quantitative method was designed to analyse the diversity of polymerase chain reaction (PCR)-amplified transcripts which possess length polymorphism. A reverse transcriptase-PCR technique was used to amplify rearranged T cell antigen receptor (TCR) transcripts isolated from human blood. Oligonucleotide primers specific for conserved TCR V and C region sequences were used in...

Topics

Base Sequence
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Clone Cells
Electrophoresis
Gene Expression
Genetic Variation
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Antigen, T-Cell, alpha-beta

Source

Identifiers

PMID: 7930651
DOI: 10.1016/0022-1759(94)90365-4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7930651-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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