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Article

Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.

Analytical biochemistry - 1 Apr 2011

Baum Paul D, Young Jennifer J, Zhang Qianjun, Kasakow Zeljka, McCune Joseph M

Abstract excerpt

Methods to measure the sequence diversity of polymerase chain reaction (PCR)-amplified DNA lack standards for use as assay calibrators and controls. Here we present a general and economical method for developing customizable DNA standards of known sequence diversity. Standards ranging from 1 to 25,000 sequences were generated by directional ligation of oligonucleotide "words" of standard length and GC content and...

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Identifiers

PMID: 21111699
DOI: 10.1016/j.ab.2010.11.035
PMCID: PMC3073996

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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