Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.

doi:10.1016/j.ab.2010.11.035

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Article

Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction.

Analytical biochemistry - 1 Apr 2011

Baum Paul D, Young Jennifer J, Zhang Qianjun, Kasakow Zeljka, McCune Joseph M

Abstract excerpt

Methods to measure the sequence diversity of polymerase chain reaction (PCR)-amplified DNA lack standards for use as assay calibrators and controls. Here we present a general and economical method for developing customizable DNA standards of known sequence diversity. Standards ranging from 1 to 25,000 sequences were generated by directional ligation of oligonucleotide "words" of standard length and GC content and...

Topics

Base Sequence
Gene Library
Genetic Variation
Molecular Sequence Data
Nucleic Acid Denaturation
Oligonucleotides
Polymerase Chain Reaction
Reference Standards
Reproducibility of Results
Sequence Analysis, DNA

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Identifiers

PMID: 21111699
DOI: 10.1016/j.ab.2010.11.035
PMCID: PMC3073996

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