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Article

Mutational analysis of residues in and around the active site of human fibroblast-type collagenase.

The Journal of biological chemistry - 21 Oct 1994

Windsor L J, Bodden M K, Birkedal-Hansen B, Engler J A, Birkedal-Hansen H

Abstract excerpt

Mutants in and around the catalytic zinc-binding site of human fibroblast-type collagenase have been expressed in Escherichia coli. Replacement of each of the three zinc ligands, His-199, His-203, and His-209, in the active site sequence: VAAHEXGHXXGXXH, not only destroyed catalytic activity but also led to improper folding of the polypeptide, suggesting that this sequence also serves as a structural zinc-binding...

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PMID: 7929334

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