Article
Critical role of glutamic acid 202 in the enzymatic activity of stromelysin-1 (MMP-3).
European journal of biochemistry - 1 Feb 2001
Arza B, De Maeyer M, Félez J, Collen D, Lijnen H R
Abstract excerpt
To test the hypothesis that Glu202, adjacent to the His201 residue that participates in the coordination of Zn(2+) in matrix metalloproteinase-3 (MMP-3 or stromelysin-1), plays a role in its enzymatic activity it was substituted with Ala, Lys or Asp by site-specific mutagenesis. Wild-type proMMP-3, proMMP-3(E202A), proMMP-3(E202K) and proMMP-3(E202D) were expressed in Escherichia coli and purified to apparent...
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