Article
Structure and mechanism of galactose oxidase. The free radical site.
The Journal of biological chemistry - 7 Oct 1994
Baron A J, Stevens C, Wilmot C, Seneviratne K D, Blakeley V, Dooley D M, Phillips S E, Knowles P F, McPherson M J
Abstract excerpt
Crystallographic and spectroscopic studies on galactose oxidase have shown that the active site involves a free radical on tyrosine 272, one of the ligands coordinated to the Cu2+ cofactor. A novel thioether bond between tyrosine 272 and cysteine 228, and a stacking tryptophan 290, over this bond...
Topics
- Base Sequence
- Binding Sites
- Crystallography, X-Ray
- Free Radicals
- Galactose Oxidase
- Kinetics
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction