Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Structure and mechanism of galactose oxidase. The free radical site.

The Journal of biological chemistry - 7 Oct 1994

Baron A J, Stevens C, Wilmot C, Seneviratne K D, Blakeley V, Dooley D M, Phillips S E, Knowles P F, McPherson M J

Abstract excerpt

Crystallographic and spectroscopic studies on galactose oxidase have shown that the active site involves a free radical on tyrosine 272, one of the ligands coordinated to the Cu2+ cofactor. A novel thioether bond between tyrosine 272 and cysteine 228, and a stacking tryptophan 290, over this bond, are features of the crystal structure. The present study describes the development of a high level heterologous...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Binding Sites
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Free Radicals

Share to Kinetics

Share to Molecular Sequence Data

Share to Mutation

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 7929198

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structure and mechanism of galactose oxidase. The free radical site.PMID 7929198Read ↗

The stacking tryptophan of galactose oxidase: a second-coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis.Semantic proximity Read ↗

Cu(I)-dependent biogenesis of the galactose oxidase redox cofactor.Semantic proximity Read ↗

Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution.Semantic proximity Read ↗

Active-site maturation and activity of the copper-radical oxidase GlxA are governed by a tryptophan residue.Semantic proximity Read ↗

Enhanced fructose oxidase activity in a galactose oxidase variant.Semantic proximity Read ↗

Crystal structures of pristine and oxidatively processed lignin peroxidase expressed in Escherichia coli and of the W171F variant that eliminates the redox active tryptophan 171. Implications for the reaction mechanism.Semantic proximity Read ↗

Elicitation of an oxidase activity in bacterial luciferase by site-directed mutation of a noncatalytic residue.Semantic proximity Read ↗

Using an artificial tryptophan "wire" in cytochrome c peroxidase for oxidation of organic substrates.Semantic proximity Read ↗

The role of distal tryptophan in the bifunctional activity of catalase-peroxidases.Semantic proximity Read ↗

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution.Semantic proximity Read ↗

Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways.Semantic proximity Read ↗

Spectroscopic evidence for an engineered, catalytically active Trp radical that creates the unique reactivity of lignin peroxidase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.