Elicitation of an oxidase activity in bacterial luciferase by site-directed mutation of a noncatalytic residue.

Article

Elicitation of an oxidase activity in bacterial luciferase by site-directed mutation of a noncatalytic residue.

The Journal of biological chemistry - 15 Mar 1990

Xi L, Cho K W, Herndon M E, Tu S C

Abstract excerpt

Flavin-dependent external monooxygenases and oxidases could catalyze the same flavin oxidation reaction involving distinct mechanisms. To gain insights into enzyme structure-function relationship, site-directed mutagenesis was carried out for Vibrio harveyi luciferase, a monooxygenase. The substi...

Topics

Catalysis
Cloning, Molecular
Flavin Mononucleotide
Kinetics
Luciferases
Luminescent Measurements
Molecular Structure
Mutation
Oxidation-Reduction
Oxidoreductases
Structure-Activity Relationship
Vibrio

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