Article
The stacking tryptophan of galactose oxidase: a second-coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis.
Biochemistry - 17 Apr 2007
Rogers Melanie S, Tyler Ejan M, Akyumani Nana, Kurtis Christian R, Spooner R Kate, Deacon Sarah E, Tamber Sarita, Firbank Susan J, Mahmoud Khaled, Knowles Peter F, Phillips Simon E V, McPherson Michael J, Dooley David M
Abstract excerpt
The function of the stacking tryptophan, W290, a second-coordination sphere residue in galactose oxidase, has been investigated via steady-state kinetics measurements, absorption, CD and EPR spectroscopy, and X-ray crystallography of the W290F, W290G, and W290H variants. Enzymatic turnover is significantly slower in the W290 variants. The Km for D-galactose for W290H is similar to that of the wild type, whereas...
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