Article
Induction of multidrug resistance (MDR) by transfection of MCF-10A cell line with c-Ha-ras and c-erbB-2 oncogenes.
International journal of cancer - 15 Oct 1994
Sabbatini A R, Basolo F, Valentini P, Mattii L, Calvo S, Fiore L, Ciardiello F, Petrini M
Abstract excerpt
To investigate the relationship between oncogene activation and appearance of multidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-10A, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erbB-2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr-1 mRNA expression, the presence of P-glycoprotein on the cell membrane and the onset...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Base Sequence
- Breast Neoplasms
- Doxorubicin
- Drug Resistance, Multiple
- Drug Screening Assays, Antitumor
- Gene Expression
- Genes, erbB-2
- Genes, ras
- Humans