Article
The role of glutamate-199 in the aging of cholinesterase.
Biochemical and biophysical research communications - 30 Nov 1993
Saxena A, Doctor B P, Maxwell D M, Lenz D E, Radic Z, Taylor P
Abstract excerpt
Aging of organophosphate-conjugated acetylcholinesterase results from the loss of an alkoxy group with concomitant stabilization of the conjugate to spontaneous or nucleophile-induced deacylation. We have examined the kinetics of aging in a pinacolylmethylphosphonofluoridate (soman)-inhibited mutant enzyme in which the glutamate (E199) located at the amino-terminal to the active-site serine (S200) was converted...
Topics
- Acetylcholinesterase
- Animals
- Cholinesterase Reactivators
- Glutamates
- Glutamic Acid
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Oximes
- Pyridinium Compounds
- Soman