Article
Subclonal heterogeneity of the multidrug resistance phenotype in a cell line expressing antisense MDR1 RNA.
Somatic cell and molecular genetics - 1 Nov 1994
Hanchett L A, Baker R M, Dolnick B J
Abstract excerpt
A multidrug resistant (MDR) cell line was transfected with an antisense MDR1 expression vector and transfectant clones were analyzed for reversion of the MDR phenotype. Only one of 10 antisense-expressing transfectants showed a reduction in drug resistance, MDR1 mRNA and P-glycoprotein. Observati...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Base Sequence
- Blotting, Northern
- Cell Nucleus
- Clone Cells
- Drug Resistance, Multiple
- Flow Cytometry
- Gene Expression
- Genetic Variation
- Microscopy, Fluorescence
- Molecular Sequence Data
- Phenotype
- RNA, Antisense
- RNA, Messenger
- Rhodamines