Article
Second site suppressor mutations of a GTPase-deficient G-protein alpha-subunit. Selective inhibition of Gbeta gamma-mediated signaling.
The Journal of biological chemistry - 30 Oct 1998
Apanovitch D M, Iiri T, Karasawa T, Bourne H R, Dohlman H G
Abstract excerpt
G proteins transmit signals from cell surface receptors to intracellular effectors. The intensity of the signal is governed by the rates of GTP binding (leading to subunit dissociation) and hydrolysis. Mutants that cannot hydrolyze GTP (e.g. GsalphaQ227L, Gi2alphaQ205L) are constitutively activat...
Topics
- Animals
- Base Sequence
- COS Cells
- Calcium-Calmodulin-Dependent Protein Kinases
- DNA Primers
- Escherichia coli
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Mating Factor
- Mutagenesis, Site-Directed
- Mutation
- Peptides
- Protein Conformation
- Saccharomyces cerevisiae
- Signal Transduction