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Article

Structure of a ricin mutant showing rescue of activity by a noncatalytic residue.

Biochemistry - 31 Mar 1992

Kim Y, Mlsna D, Monzingo A F, Ready M P, Frankel A, Robertus J D

Abstract excerpt

Ricin A chain is an N-glycosidase which removes a single adenine base from a conservative loop of 28S rRNA, thereby inactivating eukaryotic ribosomes. The mechanism of action has been proposed to include transition-state stabilization of an oxycarbonium ion on the substrate ribose by interaction with Glu 177. Conversion of Glu 177 to Gln reduces activity nearly 200-fold [Ready, M. P., Kim, Y., & Robertus, J. D....

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Identifiers

PMID: 1348187
DOI: 10.1021/bi00127a035

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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