Article
Structure of a ricin mutant showing rescue of activity by a noncatalytic residue.
Biochemistry - 31 Mar 1992
Kim Y, Mlsna D, Monzingo A F, Ready M P, Frankel A, Robertus J D
Abstract excerpt
Ricin A chain is an N-glycosidase which removes a single adenine base from a conservative loop of 28S rRNA, thereby inactivating eukaryotic ribosomes. The mechanism of action has been proposed to include transition-state stabilization of an oxycarbonium ion on the substrate ribose by interaction with Glu 177. Conversion of Glu 177 to Gln reduces activity nearly 200-fold [Ready, M. P., Kim, Y., & Robertus, J. D....
Topics
- Amino Acid Sequence
- Animals
- Artemia
- Binding Sites
- Catalysis
- Escherichia coli
- Glutamates
- Glutamic Acid
- Kinetics
- Mutation
- Ricin
- Structure-Activity Relationship