Article
Lecithin-cholesterol acyltransferase: effects of mutagenesis at N-linked oligosaccharide attachment sites on acyl acceptor specificity.
Biochimica et biophysica acta - 24 Feb 1993
Francone O L, Evangelista L, Fielding C J
Abstract excerpt
Site-directed mutagenesis was used to generate lecithin-cholesterol acyltransferase (LCAT) species in which individual attachment sites for N-linked oligosaccharide residues were replaced with residues that prevent the attachment of carbohydrate. Mutants at three of four sites retained significan...
Topics
- Amino Acid Sequence
- Binding Sites
- Cholesterol Esters
- DNA
- Escherichia coli
- Fatty Acids
- Glycosylation
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligosaccharides
- Phosphatidylcholine-Sterol O-Acyltransferase
- Phospholipases
- Polymerase Chain Reaction
- Substrate Specificity
- Transfection