Article
Two mutations of phage mu transposase that affect strand transfer or interactions with B protein lie in distinct polypeptide domains.
Journal of molecular biology - 20 May 1991
Leung P C, Harshey R M
Abstract excerpt
Two mutations within the transposase (the A protein) gene of phage Mu with distinct effects on DNA transposition have been studied. The first mutation maps to the central domain (domain II) of A, a protein consisting of three major structural domains. The variant protein is normal in synapsis and cleavage of Mu ends but is temperature-sensitive in the strand transfer reaction, joining the Mu ends to target DNA....
Topics
- Bacteriophage mu
- Chromosome Deletion
- Chymotrypsin
- Circular Dichroism
- DNA, Viral
- Escherichia coli
- Genes, Viral
- Genetic Variation
- Macromolecular Substances
- Microscopy, Electron
- Mutation