Article
Human platelet antigen-2 and -3 genotyping by PCR-SSP.
Transfusion medicine (Oxford, England) - 1 Dec 1995
Metcalfe P, Bleeker P M, von dem Borne A E, Ouwehand W H
Abstract excerpt
Allele-specific PCR using sequence specific primers (PCR-SSP) is a simple and reliable technique to detect point mutations in genes. We have developed a PCR-SSP to enable the detection of a C-T mutation at position 482 of the GPIb gene and a T-G mutation at position 13,962 in exon 26 of the GPIIb gene. These point mutations are at the basis of the HPA alloantigens 2a, 2b and 3a, 3b respectively. One primer of...
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