Article
Simultaneous DNA typing of human platelet antigens 2, 3 and 4 by an allele-specific PCR method.
Vox sanguinis - 1 Jan 1995
Tanaka S, Taniue A, Nagao N, Ohnoki S, Shibata H, Okubo Y, Yamaguchi H
Abstract excerpt
We developed an allele-specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The results were compared with those obtained by PCR restriction fragment length polymorphism and the mixed passive hemagglutination test. Seventy-three individuals were tested and the ASPCR results were in good agreement...
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