Article
Stabilization of a strained protein loop conformation through protein engineering.
Protein science : a publication of the Protein Society - 1 Mar 1995
Hodel A, Kautz R A, Fox R O
Abstract excerpt
Staphylococcal nuclease is found in two folded conformations that differ in the isomerization of the Lys 116-Pro 117 peptide bond, resulting in two different conformations of the residue 112-117 loop. The cis form is favored over the trans with an occupancy of 90%. Previous mutagenesis studies ha...
Topics
- Crystallography, X-Ray
- Genetic Variation
- Hydrogen Bonding
- Magnetic Resonance Spectroscopy
- Micrococcal Nuclease
- Models, Molecular
- Protein Engineering
- Structure-Activity Relationship
- Thermodynamics