Article
Coupling between trans/cis proline isomerization and protein stability in staphylococcal nuclease.
Protein science : a publication of the Protein Society - 1 Sept 1996
Truckses D M, Somoza J R, Prehoda K E, Miller S C, Markley J L
Abstract excerpt
The nucleases A produced by two strains of Staphylococcus aureus, which have different stabilities, differ only in the identity of the single amino acid at residue 124. The nuclease from the Foggi strain of S. aureus (by convention nuclease WT), which contains His124, is 1.9 kcal.mol-1 less stabl...
Topics
- Chemical Phenomena
- Chemistry, Physical
- Crystallization
- Enzyme Stability
- Hydrogen-Ion Concentration
- Isomerism
- Micrococcal Nuclease
- Models, Molecular
- Mutation
- Proline
- Protein Conformation
- Protein Structure, Secondary
- Solvents
- Thermodynamics
- X-Ray Diffraction