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Article

Increasing the thermostability of staphylococcal nuclease: implications for the origin of protein thermostability.

Journal of molecular biology - 20 Oct 2000

Chen J, Lu Z, Sakon J, Stites W E

Abstract excerpt

Seven hyper-stable multiple mutants have been constructed in staphylococcal nuclease by various combinations of eight different stabilizing single mutants. The stabilities of these multiple mutants determined by guanidine hydrochloride denaturation were 3.4 to 5.6 kcal/mol higher than that of the wild-type. Their thermal denaturation midpoint temperatures were 12.6 to 22.9 deg. C higher than that of the...

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Identifiers

PMID: 11023780
DOI: 10.1006/jmbi.2000.4140

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Increasing the thermostability of staphylococcal nuclease: implications for the origin of protein thermostability.PMID 11023780Read ↗

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