Accommodation of single amino acid insertions by the native state of staphylococcal nuclease.

Abstract excerpt

Single alanine and glycine insertions were introduced at 20 randomly selected positions in staphylococcal nuclease. The resulting changes in catalytic activity and in stability to guanidine hydrochloride denaturation indicate that the native state structure is frequently able to accommodate the extra residue without great difficulty, even insertions within secondary structural elements such as alpha helices and...

Topics

• Alanine
• Circular Dichroism
• Enzyme Stability
• Glycine
• Guanidines
• Micrococcal Nuclease
• Mutation
• Protein Conformation
• Protein Denaturation