Article
DNA sequence changes in mutations in the tonB gene on the chromosome of Escherichia coli K12: insertion elements dominate the spontaneous spectra.
Idengaku zasshi - 1 Feb 1995
Kitamura K, Torii Y, Matsuoka C, Yamamoto K
Abstract excerpt
To obtain insight into the nature and mechanisms of spontaneous mutations, Escherichia coli K12 strain TM31 was constructed to determine, by DNA sequencing, the mutational spectrum of the tonB gene on the chromosome. We inserted the chloramphenicol resistant gene 1.6 kb upstream of the tonB gene, thus making it possible to retrieve the mutated tonB gene from the chromosome by shotgun cloning using a...
Topics
- Base Sequence
- Chromosomes, Bacterial
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Sequence Analysis, DNA