Article
Transposition of insertion sequence IS256Bsu1 in Bacillus subtilis 168 is strictly dependent on recA.
Genes & genetic systems - 18 Oct 2017
Akashi Motohiro, Harada Shota, Moki Syunsuke, Okouji Yuki, Takahashi Kiwamu, Kada Shigeki, Yamagami Keigo, Sekine Yasuhiko, Watanabe Satoru, Chibazakura Taku, Yoshikawa Hirofumi
Abstract excerpt
We developed an insertion sequence transposition detection system called the "jumping cat assay" and applied it to the Bacillus subtilis chromosome using IS256Bsu1 derived from B. subtilis natto. The high frequency of transposition enabled us to explore host factors; combining the assay and genetic analyses revealed that recA is essential for the transposition of IS256Bsu1. Detailed analyses using various domain...
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