Article
Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase.
Applied microbiology and biotechnology - 1 Oct 1994
Hahm D H, Pan J, Rhee J S
Abstract excerpt
In order to evaluate the pta(phosphotransacetylase) (-) mutant of Escherichia coli as a potential host of foreign lipase expression, the pta(-) mutant HB101 was constructed for the purpose of blocking the acetate synthetic pathway. Since acetate is known as a major inhibitory by-product of cell growth and foreign protein production, the growth characteristics and expression kinetics of the microbial lipase of the...
Topics
- Acetates
- Acetic Acid
- Biotechnology
- Cell Division
- Colony Count, Microbial
- Culture Media
- Drug Resistance, Microbial
- Escherichia coli
- Lipase
- Mutation
- Oxidation-Reduction