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Article

Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase.

Applied microbiology and biotechnology - 1 Oct 1994

Hahm D H, Pan J, Rhee J S

Abstract excerpt

In order to evaluate the pta(phosphotransacetylase) (-) mutant of Escherichia coli as a potential host of foreign lipase expression, the pta(-) mutant HB101 was constructed for the purpose of blocking the acetate synthetic pathway. Since acetate is known as a major inhibitory by-product of cell growth and foreign protein production, the growth characteristics and expression kinetics of the microbial lipase of the...

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PMID: 7765811
DOI: 10.1007/BF00170231

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase.PMID 7765811Read ↗

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