Article
Generation of a catR deficient mutant of P. putida KT2440 that produces cis, cis-muconate from benzoate at high rate and yield.
Journal of biotechnology - 10 Dec 2011
van Duuren J B J H, Wijte D, Leprince A, Karge B, Puchałka J, Wery J, Dos Santos V A P Martins, Eggink G, Mars A E
Abstract excerpt
Pseudomonas putida KT2440-JD1 was derived from P. putida KT2440 after N-methyl-N'-nitro-N-nitrosoguanidine (NTG)-mutagenesis and exposure to 3-fluorobenzoate (3-FB). The mutant was no longer able to grow using benzoate as a sole carbon source, but co-metabolized benzoate to cis, cis-muconate during growth on glucose, which accumulated in the growth medium. The specific production rate (q(pm)) was 0.18±0.03 g cis,...
Topics
- Bacterial Proteins
- Benzoates
- Biotechnology
- DNA-Binding Proteins
- Gene Expression Regulation, Bacterial
- Mutation
- Operon
- Pseudomonas putida
- Regulatory Elements, Transcriptional
- Sorbic Acid
- Transcription Factors
- Up-Regulation