Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristic growth profiles of the mutants in a rich medium.

doi:10.1271/bbb.58.2232

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Article

Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristic growth profiles of the mutants in a rich medium.

Bioscience, biotechnology, and biochemistry - 1 Dec 1994

Kakuda H, Shiroishi K, Hosono K, Ichihara S

Abstract excerpt

Escherichia coli grown in a rich medium excreted acetate and reused the acetate. Using cloned genes and a plasmid with a temperature-sensitive replication origin, three kinds of Pta-Ack pathway deletion mutants were constructed. Acetate production and reuse by wild-type cells grown in the rich me...

Topics

Acetate Kinase
Acetates
Acetic Acid
Cloning, Molecular
Culture Media
Escherichia coli
Mutation
Phosphate Acetyltransferase
Sequence Deletion

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