Article
[High cell density culture of phosphotransacetylase mutants of Escherichia coli BL21(DE3)].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jan 2001
Zhang W C, Deng B B, Peng Q Z, Huang P T, Zhu H C
Abstract excerpt
Cell culture, organic acid production and foreign protein (TNF) expression of E. coli BL21(DE3) and its pta mutant were investigated. Under shaking conditions, TNF expression in pta mutant increased by 23%. During the fed-batch culture without limitation of specific growth rates, the mutant reached a cell density as high as 32.5 g(DCW)/L and total TNF expression at 2.8 g/L, while the parental strain only obtained...
Topics
- Acetates
- Cell Count
- Cell Culture Techniques
- Escherichia coli
- Fermentation
- Mutation
- Phosphate Acetyltransferase
- Recombinant Proteins
- Tumor Necrosis Factor-alpha