Construction of squalene-accumulating Saccharomyces cerevisiae mutants by gene disruption through homologous recombination.

doi:10.1007/BF00902741

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Article

Construction of squalene-accumulating Saccharomyces cerevisiae mutants by gene disruption through homologous recombination.

Applied microbiology and biotechnology - 1 Nov 1994

Kamimura N, Hidaka M, Masaki H, Uozumi T

Abstract excerpt

Saccharomyces cerevisiae synthesizes ergosterol via squalene, but squalene is hardly detected in aerobically grown cells. To obtain a stable squalene-accumulating yeast strain, we attempted to disrupt a gene required in the conversion of squalene to ergosterol, by homologous recombination with a...

Topics

Biotechnology
Chromosomes, Fungal
DNA, Fungal
Ergosterol
Genes, Fungal
Mutation
Recombination, Genetic
Saccharomyces cerevisiae
Squalene
Transformation, Genetic

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