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Article

Construction of squalene-accumulating Saccharomyces cerevisiae mutants by gene disruption through homologous recombination.

Applied microbiology and biotechnology - 1 Nov 1994

Kamimura N, Hidaka M, Masaki H, Uozumi T

Abstract excerpt

Saccharomyces cerevisiae synthesizes ergosterol via squalene, but squalene is hardly detected in aerobically grown cells. To obtain a stable squalene-accumulating yeast strain, we attempted to disrupt a gene required in the conversion of squalene to ergosterol, by homologous recombination with a short piece of the gene fragment conjugated with an integration plasmid vector carrying the LEU2 gene. Two mutants that...

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PMID: 7765777
DOI: 10.1007/BF00902741

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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