Article
Production and secretion of human interleukin 6 into the periplasm of Escherichia coli: efficient processing of N-terminal variants of hIL6 by the E. coli signal peptidase.
Journal of biotechnology - 1 Feb 1993
Barthelemy I, González de Buitrago G, Carreiro C, Roncal F, Pérez-Aranda A, Márquez G, Barbero J L
Abstract excerpt
We have developed a system for expressing human interleukin 6 into the periplasmic space of Escherichia coli. The method is based on the expression of the hIL6 gene under the control of the regulatory signals of plasmid pINIII-OMPA3, i.e., lpp-lac promoter and the E. coli OMPA ribosome binding site and leader sequence. Since microheterogeneity is known to occur in the amino end of the cytokine, we tested...
Topics
- Base Sequence
- Cloning, Molecular
- DNA
- Endopeptidases
- Escherichia coli
- Genetic Variation
- Humans
- Interleukin-6
- Membrane Proteins
- Molecular Sequence Data