Article
[3H]SR 48692, the first nonpeptide neurotensin antagonist radioligand: characterization of binding properties and evidence for distinct agonist and antagonist binding domains on the rat neurotensin receptor.
Molecular pharmacology - 1 May 1995
Labbé-Jullié C, Botto J M, Mas M V, Chabry J, Mazella J, Vincent J P, Gully D, Maffrand J P, Kitabgi P
Abstract excerpt
The binding of [3H]SR 48692, a new potent and specific nonpeptide neurotensin (NT) receptor antagonist, was characterized in membranes from mouse fibroblast LTK- cells stably transfected with the G protein-coupled rat NT receptor. The binding of [3H]SR 48692 was specific, time dependent, reversib...
Topics
- Animals
- Binding Sites
- Binding, Competitive
- Cell Line
- Digitonin
- Guanosine 5'-O-(3-Thiotriphosphate)
- Kinetics
- Mice
- Mutation
- Pyrazoles
- Quinolines
- Radioligand Assay
- Rats
- Receptors, Neurotensin
- Sodium