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Article

New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites.

Journal of cell science - 1 May 1999

Belmont L D, Patterson G M, Drubin D G

Abstract excerpt

We have generated 9 site-specific mutations in Saccharomyces cerevisiae actin. These mutants display a variety of phenotypes when expressed in vivo, including slow actin filament turnover, slow fluid-phase endocytosis, and defects in actin organization. Actin mutation D157E confers resistance to the actin-sequestering drug, latrunculin A. Latrunculin A inhibits nucleotide exchange on wild-type yeast actin but not...

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PMID: 10194411
DOI: 10.1242/jcs.112.9.1325

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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