Article
Interleukin-1 beta converting enzyme requires oligomerization for activity of processed forms in vivo.
The EMBO journal - 1 May 1995
Gu Y, Wu J, Faucheu C, Lalanne J L, Diu A, Livingston D J, Su M S
Abstract excerpt
Interleukin-1 beta converting enzyme (ICE) is composed of 10' (p10) and 20 kDa (p20) subunits, which are derived from a common 45 kDa precursor. Recent crystallographic studies have shown that ICE exists as a tetramer (p20/p10)2 in the crystal lattice. We provide evidence that the p10 and p20 subunits of ICE associate as oligomers in transfected COS cells. Using intragenic complementation, we show that the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Caspase 1
- Cell Line
- Cysteine Endopeptidases
- DNA, Complementary
- Genetic Complementation Test
- Humans
- Models, Molecular