Article
Detection of protein kinase homologues and viral RNA-binding domains utilizing polyclonal antiserum prepared against a baculovirus-expressed ds RNA-activated 68,000-Da protein kinase.
Virology - 1 Dec 1992
Barber G N, Tomita J, Garfinkel M S, Meurs E, Hovanessian A, Katze M G
Abstract excerpt
The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation factor 2 (elF-2), leading to potential limitations in functional elF-2 and decreases in protein...
Topics
- Baculoviridae
- Base Sequence
- Enzyme Activation
- Gene Expression Regulation, Enzymologic
- Humans
- Molecular Sequence Data
- Mutation
- Protein Serine-Threonine Kinases
- RNA, Double-Stranded
- RNA, Viral