Article
A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990
Kaye F J, Kratzke R A, Gerster J L, Horowitz J M
Abstract excerpt
We have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative...
Topics
- Amino Acid Sequence
- Base Sequence
- Carcinoma, Small Cell
- Cell Line
- DNA, Neoplasm
- DNA-Binding Proteins
- Genetic Vectors
- Humans
- Lung Neoplasms
- Molecular Sequence Data
- Mutation
- Phosphoproteins
- Phosphorylation
- Plasmids
- Polymerase Chain Reaction
- RNA, Messenger
- Restriction Mapping
- Retinoblastoma Protein