Article
Restriction isotyping of apolipoprotein E R145C in type III hyperlipoproteinemia.
Journal of investigative medicine : the official publication of the American Federation for Clinical Research - 1 Apr 1995
Hsia S H, Connelly P W, Hegele R A
Abstract excerpt
BACKGROUND: An adolescent female who presented with type III hyperlipoproteinemia was found to have an E3/2 phenotype by isoelectric focusing while restriction isotyping using HhaI revealed a pattern compatible with classical E3/3. Studies were carried out to determine the nature of the patient's apolipoprotein E abnormality. METHODS: A 244 bp fragment of exon 4 of apolipoprotein E was amplified by the polymerase...
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