Article
A comprehensive system to explore p53 mutations.
American journal of clinical pathology - 1 Sept 1998
Furuwatari C, Yagi A, Yamagami O, Ishikawa M, Hidaka E, Ueno I, Furihata K, Ogiso Y, Katsuyama T
Abstract excerpt
To establish an effective and reliable system for the detection of p53 mutations, we evaluated the detection efficiencies of nonisotopic polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP), fluorescence in situ hybridization (FISH), and immunohistochemistry. Ten cell line...
Topics
- Cell Line
- DNA Mutational Analysis
- DNA, Neoplasm
- Evaluation Studies as Topic
- False Positive Reactions
- Genes, p53
- Humans
- Immunoenzyme Techniques
- In Situ Hybridization, Fluorescence
- Loss of Heterozygosity
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Sequence Analysis, DNA
- Tumor Suppressor Protein p53