Article
Depletion of c-myc with specific antisense sequences reverses the transformed phenotype in ras oncogene-transformed NIH 3T3 cells.
Molecular and cellular biology - 1 Jul 1991
Sklar M D, Thompson E, Welsh M J, Liebert M, Harney J, Grossman H B, Smith M, Prochownik E V
Abstract excerpt
ras oncogene-transformed NIH 3T3 cells expressing glucocorticoid-inducible antisense c-myc cDNA transcripts at levels sufficient to deplete c-myc protein lost their transformed morphology and the ability to grow in soft agar; their ability to form tumors in nude mice was also impaired. These changes were dependent on the continuous expression of the antisense sequences. No major effects on plating efficiencies,...
Topics
- Animals
- Blotting, Southern
- Cell Cycle
- Cell Division
- Cell Line
- Cell Transformation, Neoplastic
- Chromosome Deletion
- DNA, Antisense
- Genes, myc
- Genes, ras
- Genetic Vectors