Article
Random mutagenesis used to probe the structure and function of Bacillus stearothermophilus alpha-amylase.
Protein engineering - 1 Jan 1990
Holm L, Koivula A K, Lehtovaara P M, Hemminki A, Knowles J K
Abstract excerpt
Mutations that cover the sequence of Bacillus stearothermophilus alpha-amylase were produced by an efficient in vitro enzymatic random mutagenesis method and the mutant alpha-amylases were expressed in Escherichia coli, which also secreted the product. Ninety-eight mutants were identified by sequ...
Topics
- Amino Acid Sequence
- Binding Sites
- Calcium
- Geobacillus stearothermophilus
- Models, Molecular
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Protein Conformation
- Sequence Homology, Nucleic Acid
- Structure-Activity Relationship