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Article

Random mutagenesis used to probe the structure and function of Bacillus stearothermophilus alpha-amylase.

Protein engineering - 1 Jan 1990

Holm L, Koivula A K, Lehtovaara P M, Hemminki A, Knowles J K

Abstract excerpt

Mutations that cover the sequence of Bacillus stearothermophilus alpha-amylase were produced by an efficient in vitro enzymatic random mutagenesis method and the mutant alpha-amylases were expressed in Escherichia coli, which also secreted the product. Ninety-eight mutants were identified by sequencing and their enzyme activities were classified into three classes: wild-type, reduced or null. A molecular model of...

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PMID: 2330367
DOI: 10.1093/protein/3.3.181

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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