Generation of a temperature-sensitive cSRC.

Abstract excerpt

We have created retroviruses encoding a temperature-sensitive variant of the cSrc protein, and of its activated allele cSrc(Y527F). Cells expressing the activated allele harboring this mutation displayed a transformed phenotype when grown at 34 degrees, but not at 39 degrees, as quantitated by measuring the susceptibility of the cells to contact inhibition and their ability to grow anchorage-independently at the...

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