Analysis of second-site revertants of a murine coronavirus nucleocapsid protein deletion mutant and construction of nucleocapsid protein mutants by targeted RNA recombination.

Abstract excerpt

The Alb4 mutant of the coronavirus mouse hepatitis virus (MHV) is both temperature sensitive and thermolabile owing to a deletion in the gene encoding its nucleocapsid (N) protein. The deletion removes 29 amino acids that constitute a putative spacer region preceding the carboxyl-terminal domain...

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Capsid
• Cell Line
• Mice
• Molecular Sequence Data
• Murine hepatitis virus
• Mutation
• RNA, Viral
• Recombination, Genetic
• Sequence Deletion
• Viral Core Proteins