Mutations in v-Src SH3 and catalytic domains that jointly confer temperature-sensitive transformation with minimal temperature-dependent changes in cellular tyrosine phosphorylation.

Abstract excerpt

We have analyzed two functionally significant amino acid alterations encoded by the temperature-sensitive (ts) v-src mutant of Rous sarcoma virus, LA32. The G-to-V change at residue 300 in the catalytic domain nonconditionally impairs morphological transformation, in vitro kinase activity, in viv...

Topics

• Animals
• Avian Sarcoma Viruses
• Catalysis
• Cell Transformation, Viral
• Cells, Cultured
• Chick Embryo
• Mutation
• Oncogene Protein pp60(v-src)
• Phosphatidylinositol 3-Kinases
• Phosphorylation
• Phosphotransferases (Alcohol Group Acceptor)
• Temperature
• Tyrosine